Apa I
| Catalog # | Pack size | Price, Euro | SHOP |
| 209010 | 1000 u | 16,00 | Add to cart |
| 209050 | 5000 u | 64,00 | Add to cart |
Download: Datasheet Apa I
GGGCC↓C
C↑CCGGG
Purified from Acetobacter pasteurianus
Concentration:
10-30000 units/ml
Activity assay:
Phage λ DNA(dam-)/BamH I digest, SE buffer Y, 37oC
Recommended buffer:
Y
Ligation/recutting assay:
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 40 units of enzyme at 37oC.
Heat inactivation (20 min, 65oC):
Yes
Note! Cleavage by Apa I may be blocked by overlapping dcm-methylation
