Acc16 I

TGC↓GCA

ACG↑CGT

Purified from Acinetobacter calcoaceticus 16

Isoschizomers Fsp I, Nsb I

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 37^oC

Recommended buffer:
W

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of enzyme at 37^oC.

Heat inactivation (20 min, 65^oC):
Yes

Note! Activity may be blocked by overlapping dcm methylation.

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