• Start Bioron International
• Hot-Start PCR
• PCR Products
• Devices
• Nucleotides
• DNA / Markers
• DNA-RNA Purification
• Other Enzymes
• Dialysis Membranes
• Plastic Consumables
Excellent Products from Bioron

• PCR Products
• Real time PCR
• Devices
• Diagnostic Kits
• DNA Marker, DNA, Plasmid
• DNA-RNA Purification
• Protein Purification
• Nucleotides
• Dialysis Membranes
• Other Enzymes
• Mol-Bio Chemicals
  • Proteinase K
  • Agarose
  • X-gal (MBG)
  • RNAse mimic
  • IPTG (MBG)
  • Linear Polyacrylamide
• Restriction Enzymes
• Mutation Analysis
• Tubes, tips, Tissue culture
• Advertising 07/08
• Distribution

Download
Catalog 2007

RNAse mimic

R-mim^TM,   0.1 mM solution in water  

Datasheet: RNase mimic

RNAse mimic is a chemically synthesized organic molecule capable to cleave RNA in RNAseA-like manner (1)

.

R-mim^TM molecule designed so, that it imitates the action of RNAseA, digesting RNA mainly at CpA and UpA sequences in single-stranded stretches of RNA. Due to the low molecular weight R-mim^TM may penetrate the secondary structure of RNA, RNA-protein complexes, viral particles and living cells. As compared to enzymes, it withstands a wide range of conditions and do not perturb the object of action. In contrast to other RNAse-mimetics, R-mim^TM doesn't contain metal ions and therefore does not inhibit enzymes used in molecular biology manipulations (Taq DNA polymerase, restriction endonucleases, polynucleotidekinase etc.).

MW: 693.60

Purity: 95% (H1-NMR)

R-mim^TM chemical structure:    

R-mim^TM  gives only highly reproducible results!

R-mim^TM  has several advantages on the natural RNAseA preparations, namely:

• no unspecific impurities.
• no ballast protein.
• specific activity does not vary from lot to lot.
• the preparation is pure from other RNAses; very high stability; no inhibition by heating, high salt concentrations (including GuSCN), organic impurities (including phenol).
• no degradation by proteases.
• easy to separate from proteins, DNA, RNA.

Use R-mim^TM for:

• the study of RNA secondary structure
• the study of RNA-protein interaction.
• the cleavage of RNA within the cell, viral particles, RNA-protein complexes.
• digestion of RNA in bulk quantities.

1 molecule of R-mim^TM catalyzes the digestion of 150-170 of phosphodiether bonds in RNA for 60 min at 25°C. We recommend using 0,01 mM concentration of R-mim in your experiments.

Storage and shipment:
room temperature

ATTENTION: DO NOT FREEZE THE PRODUCT    

The following points are to be considered when RNase mimic is used:
- the concentration of RNA should not be higher than 0,5mg/ml;
- the majority of tests with RNA digestions were done on purified tRNA and viral RNA (both successful), not too much experimets were performed with other types of RNA;
- presence of excess of small RNA molecules may reduce strongly the effectivity of digestion.

Reference: 1. Giege R. at al. (2000), Methods in Enzymology, v.318, p.147

Copyright © BIORON GmbH