Linear Polyacrylamide
Linear Polyacrylamide Solution
Catalog # | Pack size | Price, Euro | SHOP |
| 130101 | 1 ml | 12,00 | Add to cart |
| 130105 | 1 ml x 5 | 50,00 | Add to cart |
Datasheet: Linear Polyacrylamide
Description:
Linear polyacrylamide is used as an additive to DNA or RNA solutions for the improvement of nucleic acids recovery during alcohol precipitation. Fragments larger than 20 bases can be precipitated in picogramm amounts, while nucleotides and oligonucleotide primers remain in the liquid phase. Linear Polyacrylamide is not produced from biological material, contacted neither enzymes nor other biomolecules. Due to this feature, Linear Polyacrylamide is the most appropriate co-precipitant of DNA or RNA for the subsequent PCR/RT-PCR.
Quality control :
Endonuclease/nickase activity is not detected after 16 incubation at 37oC of 5 microgramm of Linear Polyacrylamid with 0,5 microgramm of supercoiled pUC19 plasmid DNA.
Concentration: 5 mg/ml, solution in water
Storage: -20oC
Shipment: ambient temperature
Reference:
Gillard, C. and Strauss F. Nucleic Acid Research, Vol. 18. No. 2, p. 378, 1990.
Protocol for DNA precipitation with Linear Polyacrylamide as co-precipitant
- Add linear polyacrylamide to DNA solution to the final concentration of 10-20 µg/ml, mix well.
- Add one volume of isopropanol or 2 volumes of ethanol.
- Chill for 15 min in freezer (-20oC), centrifuge at < 10,000 g for at least 15 min.
- Carefully remove supernatant and resuspend the pellet in an appropriate buffer.
