Bgl I
| Catalog # | Pack size | Price, Euro | SHOP |
| 250105S | 2.000 units | 20,00 | Add to cart |
| 250105L | 10.000 units | 98,00 | Add to cart |
Cutting Sequence: GCCNNNN↓NGGC
Isoschizomere: N/A
Source: Bacillus globigii lacking BglII.
Buffer supplied: 10x Bgl I.
Substrate for unit definition: λ DNA.
Reaction conditions: 50 mM NaCl, 100 mM Tris-HCl (pH 7.9 at 25°C), 5 mM MgCl2, 0.025% Triton X-100, 100 μg/ml BSA. Incubate at 37°C.
Storage buffer: 200 mM NaCl, 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.
Absence of contaminants: Fifty units of BglI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After ten-fold overdigestion with BglI, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes.
Reaction Buffer Table
