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Dry PCR Master Mix (no-stain)

Catalog #Pack sizePrice, EuroSHOP
116101100 tubes (100 rcs)160,00Add to cart

For research use only

Datasheet: Dry PCR Master Mix (no-stain)

THE TUBES MUST BE STORED AND SHIPPED AT ROOM TEMPERATURE

The qPCR GenePack Tubes kit contains

1.   100 Master Mix tubes (0,2 ml PCR tubes with optical caps for ABI- and BioRad-Cycler models).  Each Master Mix tube contains lyophilized mixture of "Hot-Start" Taq DNA polymerase for "QPCR" heat-sensitive Taq DNA polymerase inhibitors, dNTPs, MgCl2 all in amounts adjusted for optimal "Hot-Start" PCR in 25 µl volume.

2.   PCR diluent

Protocol for PCR with DryMix SuperHot Taq DNA Pol

  • Take the required amount of Master Mix tubes, label them according to the plan of your experiment. Always use 1 tube as positive control (template DNA) and one tube as negative control (no template DNA) in all your experiments
  • Centrifuge tubes briefly to place the lyophilized mixture on the bottoms of the tubes
  • Add 10 µl of PCR diluent in each tube
  • Add 5 µl of primer mix in each tube (recommended concentration - 0.1-0.5 mM of each)
  • Add 5 µl of sample (template DNA) in each tube
  • Centrifuge tubes briefly to get rid of possible air bubbles in the mixture
  • Run the PCR in thermocycler as usual.
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