DFS-Taq DNA Polymerase
| Catalog # | conc. | Pack size | Price, Euro | SHOP |
| 101005 | 5,0u/µl | 500 u | 49,00 | Add to cart |
| 101025 | 5,0u/µl | 2500 u | 196,00 | Add to cart |
| 101100 | 5,0u/µl | 10000 u | 549,00 | Add to cart |
| 101500 | 5,0u/µl | 50000 u | 1921,50 | Add to cart |
Applications
DFS (DNA Free Sensitive) Taq DNA Polymerase is suitible for:
- Standard PCR of DNA fragments up to 5 kb.
- Realtime PCR.
- Primer extension reaction.
- PCR with bacterial DNA.
Description
DFS (DNA Free Sensitive) Taq DNA Polymerase is a thermostable DNA Polymerase of approximately 94 kDa isolated from eubacterium Thermus aquaticus strain YT-1(1). This unmodified Taq DNA Polymerase replicates DNA at 72°C. The DFS-Taq Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5'--> 3' direction in the presence of magnesium ions and possesses a 5'--> 3' exonuclease activity. The DNA-free Taq Polymerase is highly purified and is free of nonspecific endo- or exonucleases. Taq DNA Polymerase leaves single 3'-dA nucleotide overhangs on their reaction products.
Features
- This Taq DNA Polymerase is absolutely free from
bacterial DNA.
- With Bioron Buffer system for all purposes suitable.
- High sensitivity: 6 molecules template may be enough.
- Efficient amplification of single copy genes.
- High yields of products.
Concentration: 5 units/µl
Storage conditions: -20oC
E. coli DNA Test with DFS- and another Taq Polymerase
using E.coli 16S RNA specific primers:
1 - without adding E.coli DNA (Test)
2 - with adding E.coli DNA (control)
Reference: 1. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya 45, 644(Rus)
