| Catalog # | Pack size | Price, Euro | SHOP |
| 131002 | 200 units | 75,00 | Add to cart |
| 131010 | 1000 units | 299,00 | Add to cart |
Datasheet: GC-rich PCR DNA Polymerase
Description: GC-rich PCR DNA Polymerase is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp in lenght with superior accuracy and yield. The enzyme's 3'-> 5' exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than other commercially available DNA polymerase. The elongation rate and processivity are 5 times and 10 to 15 times higher, respectively, than for Pfu DNA Polymerase, resulting in highly accurate and robust yields in short reaction times. The enzyme has no 5'-> 3' exonuclease activity and generates blunt-ended PCR products suitable for cloning
| GC-rich | Pfu | Taq | |
| Species | Thermococcus kodakaraensis | Pyrococcus furiosus | Thermus aquaticus |
| Fidelity | 0,0035 | 0,0039 | 0,013 |
Elongation rate (bases/second) | 106-138 | 25 | 61 |
Processivity (nucleotide bases) | >300 | <20 | not determined |
Qualitycontrol: It is designed for accurate amplification of complex, GC-rich target DNA. The enzyme was tested on the absence of endonuclease – and exonuclease activities.
Storage Buffer: 50 mM Tris-HCl (pH 8.0); 50 mM KCl; 1 mM DTT; 0.1 mM EDTA; stabilizers; and 50% (v/v) glycerol
Reaction buffer GC-rich (10x): 1.2 M Tris-HCl (pH 8.0); 100 mM KCl; 60 mM (NH4)2SO4; 1% Triton X-100; 0.01% BSA. Plus an extra Tube of: 25 mM MgCl2
Storage condition: Recommended storage temperature is -20°C.
Concentration: 5 u/µl
Unit definition: One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl2, 0,5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.
