| Catalog # | Pack size | Price, Euro | SHOP |
| 401001 | 500 µg | 99,00 | Add to cart |
Description:
COT I Human DNA is prepared exclusively from male human placental DNA by shearing, denaturing, and reannealing under conditions that enrich repetitive elements. The COT I fraction of human genomic DNA consists largely of rapidly annealing repetitive elements.
Quality Control:
Avarage fragments size - 50-300 bp;
A260/A280: 1.78;
amount of genomic (non-repetitive DNA) - 2%.
The sample as well as original material were tested for the absence of HIV1,2 RNA, HCV RNA, HBV DNA.
Concentration:
>1 mg/ml
Applications:
COT I Human DNA can be used for suppressing crosshybridization to human repetitive DNA in filter and microarray hybridizations and in situ hybridization experiments. Repetitive elements present in a probe may result in unspecific hybridization signals. To enable specific hybridization of the probe to the chromosomal target site the probe is denatured together with an excess of unlabeled COT I Human DNA as a competitor. As a result single-cope gene hybridization is not hindered by repetitive sequences.
Storage: -20 °C
