PCR Endpoint Reader
| Catalog # | Pack size | Price, Euro | SHOP |
| 900001 MX 2,6 (2 channels, USB) | 1 unit | on request | Add to cart |
| 900010 4 CL (4 channels, USB) | 1 unit | on request | Add to cart |
Manual of GeneMachineThe Gene-Machine is: 
- performed for fluorescence detection in regular PCR tubes, 0,6 ml for Cat-no: 900001). For Cat-No: 900010, 0,6 ml and 0,2 ml Standart-PCR Tubes.
- intended for the use of probes and-or primers labeled by a fluorescent label and quencher
- allows to register PCR results after the end of reaction without any opening of a test-tube - contamination risk is minimized and the requirements to the PCR diagnostic laboratory organization are simplified
- reduces considerably the hand-work and the duration of the PCR detection stage
- an efficient documentation system of PCR results
- a comfortable replacement for Agarose gels, UV-Tables; a time saveing system
- together ideal software for documentation
- ideal for diagnostic applications (yes/no statements)
Standard investigated material:
Blood, Female urogenital duct epithelial scrab, Male urethra epithelial scrab, Prostate gland secret, Sperm, Urine, Faeces, Lachrymal liquid, Biopsy and autopsy materials, Conjunctiva smear, Fauces smear, Fauces lavage, Phlegm, Bronchial alveole lavage, Bronchus lavage, Saliva, Lesion’s smears and lavages, Milk
Technical parameters:
- This device is intended for the registration of PCR results obtained with the use of test systems based on fluorescent detection principles
- Number of detection channels:
cat-no: 900001: 2 channels
cat-no: 900010: 4 channles - Tube volume:
cat-no: 900001: 0,6 ml
cat-no: 900010: 0,6 ml and 0,2 ml stadard-PCR tubes - Excitation/emission wavelengths:
cat-no: 900001: 470/514; 532/580 nm
cat-no: 900010: 460/520; 532/570; 580/620; 632/675 nm - Time of detection for one block (12 tubes):
cat-no: 900001: 15 seconds
cat-no: 900010: 30 seconds - Interface type: USB 2.0
- software
The machine provides a technology between Real Time and regular PCR. A lot of PCR applications aimed to answer the question if the target template can be found in the sample or not without need for the exact quantitation. This is true for the majority of Infection-disease pathogen detection systems.
The "end-point detection" PCR technique designed for fluorescent probe is a simple and economic alternative to the Real-Time PCR approach. There is no possibility for quantitation.
The end-point PCR d
etection provides all other advantages of Real Time PCR - the simplicity of result detection, possibility to store the result as the files on the computer, the possibility not to handle the samples after PCR.
The "end-point detection" PCR technique can utilize the same chemistry as Real Time PCR - Taq Man assay (under patent no: EP 543 942 and EP 919 565 and other relevant patent of Roche Diagnostics), Molecular Beacons, Scorpion etc. PCR is performed in the regular PCR cycler.
After the PCR, tubes are transferred to the "Gene" detector. "Gene" detector provides the measurement of fluorescence directly in PCR tubes. 12 tubes can be loaded into the machine simultaneously; the best is to run multiplex PCR in each tube - control PCR (which should be always positive) and test (sample)-PCR. The machine provides such a possibility since 2 channels are available - for excitation at 470 nm with fluorescent measurement at 514 nm and for excitation at 532 nm with fluorescent measurement at 580 nm. (For cat-no: 900010: 460/520; 532/570; 580/620; 632/675 nm)
SOFTWARE OF GENE-MACHINE
"Gene-Machine" is the only available on the market fluorimeter wich can measure the fluorescence directly in regular plastic tubes, the software of the device is simple and user-friendly, and the machine is several times cheaper than the cheapest fluorimeter available on the market
